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KMID : 0191120210360090064
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Journal of Korean Medical Science
2021 Volume.36 No. 9 p.64 ~ p.64
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Comparison of Serologic Response of Hospitalized COVID-19 Patients Using 8 Immunoassays
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Lee Sun-Min
Kim In-Suk
Lim Seung-Jin
Lee Su-Jin
Kim Won-Joo
Shin Kyung-Hwa
Moon Soo-Young
Chang Chul-Hun L.
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Abstract
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Background: In Korea, there were issues regarding the use of immunoassays for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies to detect infection. So, we compared antibody results of eight kinds of commercial immunoassays using clinical remnant specimens.
Methods: We compared the results of several immunoassay kits tested on 40 serum samples from 15 confirmed patients and 86 remnant serum samples from clinical laboratory. Eight kinds of IVD kits?four enzyme-linked immunosorbent assay, two lateral flow rapid immunochromatographic assays, and two chemiluminescent immunoassays with one RUO kit were tested.
Results: Among 40 serum samples from 15 coronavirus disease 2019 (COVID-19) patients, 35 yielded at least one positive result for detecting antibodies in the combined assessment. There were inconsistent results in 12 (28%) samples by single immunoassay. Forty samples collected in 2019 before the first COVID-19 Korean case showed negative results except for one equivocal result.
Conclusion: The discrepant results obtained with different immunoassay kits in this study show that serological assessment of SARS-CoV-2 by a single immunoassay requires caution not only in detecting infection but also in assessing immunologic status.
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KEYWORD
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SARS-CoV-2, COVID-19, ELISA, Immunoassay, Korea, Antibodies
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